Each graduation mark equals an increment of two one-hundredths (0.02 μl) of a microliter. The third number in red indicates tenths of a microliter (0.1 μl). The second number in black denotes the volume in microliters. The top number in black is for tens of microliters this should only be set at "2" with the other two numbers set at "0" when dispensing 20.0 μl. The third number indicates tenths of a microliter (0.1 μl). The middle number denotes volume in microliters. The top number is for tens of microliters this usually is set at "0" and should only be set at "1" with the other two numbers set at "0" when dispensing 10.0 μl. Each graduation mark equals an increment of two one-thousandths (0.002 μl) of a microliter. The second number indicates tenths of a microliter (0.1 μl), and the third number represents hundredths of a microliter (0.01 μl). The top number denotes volume in microliters. Note that each micropipettor is only as accurate as the smallest graduation mark. Depending on the micropipettor, the numbers are interpreted differently. Then slowly dial down the thumb wheel to reach the intended volume, making sure not to overshoot the graduation mark. To obtain maximum accuracy when increasing the volume setting on the micropipettor, dial the thumb wheel up, passing the desired graduation mark by 1/3 of a turn.
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